IGF1 Receptor

The IGF1 receptor is highly related in sequence and structure to the insulin receptor, but has distinct biological functions, one of which is cell survival. Therefore, this RTK is a potential target for inhibition in tumor cells. In collaboration with Dr. Todd Miller at SUNY-Stony Brook, we have determined the three-dimensional structure of the IGF1 receptor kinase domain using x-ray crystallography. Several amino acid differences between the IGF1 receptor and the insulin receptor near the ATP binding cleft might be exploited by small-molecule inhibitors to gain selectivity for the IGF1 receptor over the insulin receptor. To this end, structural studies of inhibitors bound to the IGF1 receptor kinase are being pursued.

 

Structure of the kinase domain (light and dark gray) of the IGF1 receptor in complex with a small-molecule inhibitor. The ATP-competitive inhibitor is shown in (semi-transparent) sphere representation. The kinases formed a crystallographic dimer in which a tyrosine in the activation loop (green) is bound in the active site (orange) of the other kinase domain (and vice versa), providing a view of trans-autophosphorylation in the act. [Wu et al., EMBO J. 27, 1985-1994 (2008)]